Curriculum Vitae: Xiaowei Zhuang
Professor of Chemistry and Chemical Biology
Professor of Physics
Howard Hughes Medical Institute Investigator
12 Oxford Street, Cambridge, MA 02138
Tel: (617) 496-9558 Fax: (617) 496-9559
Email: zhuang@chemistry.harvard.edu Website: http://zhuang.harvard.edu
Education
1987-1991 B.S., Physics, University of Science and Technology of China
1991-1996 Ph.D., Physics, University of California, Berkeley
1997-2001 Chodorow Postdoctoral Fellow, Stanford University
Professional Appointments
09/2005- Investigator, Howard Hughes Medical Institute
01/2006- Professor, Department of Chemistry and Chemical Biology,
Department of Physics, Harvard University
07/2005-12/2005 Associate Professor, Department of Chemistry and Chemical Biology,
Department of Physics, Harvard University
08/2001-06/2005 Assistant Professor, Department of Chemistry and Chemical Biology,
Department of Physics, Harvard University
Honors and awards
2006 Pure Chemistry Award
2005 Camille Dreyfus Teacher-Scholar Award
2004 Alfred P. Sloan Research Fellowship
2004 MIT Technology Review Worlds Top 100 Young Innovators Award
2003 MacArthur Fellowship
2003 Packard Fellowship for Science and Engineering
2003 CAREER award, National Science Foundation
2003 Searle Scholarship
2003 Beckman Young Investigator Award
2002 Young Investigator Award, Office of Naval Research
2000 National Research Service Award, National Institute of Health
1997 Chodorow Postdoctoral Fellowship, Stanford University,
1993 Victor F. Lenzen Memorial Scholarship, University of California
1992 George C. and Helen N. Pardee Scholarship, University of California
1991 Bernice Teuteberg Memorial Scholarship, University of California
Professional Services
Co-Chair, American Chemical Society Symposium on ““Single-molecule imaging, spectroscopy, and
manipulation of biomolecular systems,” (2007)
Vice Chair, Gordon Research Conference on “Single Molecule Approaches to Biology,” (2006)
Editorial Board, Annual Review on Biophysics and Biomolecular Structure (2006 - )
Adhoc Member, NIH study section on cell structure and function (2006)
Member, NIH study section, Nanomedicine Development Centers for the NIH Roadmap Nanomedicine
Initiative (2005)
Member, steering committee, APS workshop on "Opportunities in Biology for Physicists" (2005)
Member, Steering committee, Broad Institute, Harvard University, (2003 – 2006)
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Member, Standing committee, Biophysics program, Harvard University, (2002 - )
Member, Executive committee, Center for Imaging and Mesoscopic Science, Harvard University (2002-
2004)
Adhoc Member, NIH study section, BMCE (2003)
Member, steering committee, APS workshop on "Opportunities in Biology for Physicists" (2002)
Professional Memberships
American Biophysical Society, American Chemical Society, American Physical Society, American
Society of Cell Biology,
Submitted Manuscripts and Publications
1. M. D. Stone, M. Mihalusova, C. M. O’Connor, R. Prathapam, K. Collins, X. Zhuang, “Stepwise
protein-mediated RNA folding directs assembly of telomerase ribonucleoprotein,” Nature (in press).
2. B. Brandenburg, X. Zhuang, “Virus trafficking, learning from single-virus tracking,” Nat. Rev.
Microbiology (in press).
3. C. K. Payne, S. Jones, C. Chen, X. Zhuang, “Internalization and trafficking of cell surface
proteoglycans and proteoglycan binding ligands,” Traffic (in press).
4. Y. Zhou and X. Zhuang, “Robust reconstruction of the rate constant distribution using the phase
function method,” Biophys. J. 91, 4045-4053 (2006)
5. M. J. Rust, M. Bates, X. Zhuang, “Sub-diffraction-limit imaging by stochastic reconstruction optical
microscopy (STORM),” Nature Methods 3, 793-795 (2006)
6. G. Bokinsky, L. G. Nivon, S. Liu, G. Chai., M. Hong, K. M. Weeks, X. Zhuang, “Two distinct
binding mode of a protein cofactor with its target RNA,” J. Mol. Biol. 361, 771-784 (2006)
7. M. Lakadamyali, M. J. Rust, X. Zhuang, “Ligands for clathrin-mediated endocytosis are
differentially sorted into distinct populations of early endosomes,” Cell 124, 997-1009 (2006)
8. X. Zhuang, “Single-molecule RNA Science,” Annu. Rev. Biophys. Biomol. Struct. 34, 399-414
(2005)
9. G. Bokinsky, X. Zhuang, “Single-molecule RNA folding,” Acct. Chem. Res. 38, 566-573 (2005)
10. M. Bates, T. R. Blosser, X. Zhuang, “Short-range spectroscopic ruler based on a single-molecule
optical switch” Phys. Rev. Lett. 94, 101801 (2005)
11. X. Zhuang, “Unraveling DNA condensation by optical tweezers,” Science 305, 188-190 (2004)
12. M. J. Rust, M. Lakadamyali, F. Zhang, X. Zhuang, “Assembly of endocytic machinery around
individual influenza viruses during viral entry,” Nature Struct. Mol. Biol. 11, 567-573 (2004)
13. M. Lakadamyali, M. J. Rust, X. Zhuang, “Endocytosis of influenza viruses,” Micro. Infect. 6, 929-
936 (2004)
14. F. Patolsky, G. Zheng, O. Hayden, M. Lakadamyali, X. Zhuang, C. M. Lieber, “Electrical detection
of single viruses”, Proc. Natl. Acad. Sci. USA 101, 14017-14022 (2004)
15. H. P. Babcock, C. Chen, X. Zhuang, “Using single-particle tracking to study nuclear trafficking of
viral genes,” Biophys. J. 87, 2749-2758 (2004)
16. D. Rueda, G. Bokinsky, M. M. Rhodes, M. J. Rust, X. Zhuang, N. G. Walter, “Single-molecule
enzymology of RNA: Essential functional groups impact catalysis from a distance,” Proc. Natl.
Acad. Sci. USA 101, 10066-10071 (2004)
17. M. Lakadamyali, M. J. Rust, H. P. Babcock, X. Zhuang, “Visualizing infection of individual
influenza viruses,” Proc. Natl. Acad. Sci. USA 100, 9280-9285 (2003)
18. G. Bokinsky, D. Rueda, V. K. Misra, A. Gordus, M. M. Rhodes, H. P. Babcock, N. G. Walter, X.
Zhuang, “Single-molecule transition-state analysis of RNA folding,” Proc. Natl. Acad. Sci. USA
100, 9302-9307 (2003)
19. X. Zhuang and M. Rief, “Single-molecule folding,” Curr. Opin. Struct. Biol. 13, 88-97 (2003)
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20. L. E. Bartley, X. Zhuang, R. Das, S. Chu, D. Herschlag, "Exploration of the transition state for
tertiary structure formation between an RNA helix and a large structured RNA," J. Mol. Biol. 328,
1011-1026 (2003)
21. X. Zhuang, H. Kim, M. Pereira, H. Babcock, N. Walter, S. Chu, “Correlating structural dynamics
and function in single ribozyme molecules,” Science 296, 1473-1476 (2002)
22. R. Russell, X. Zhuang, H. Babcock, I. S. Millett, S. Doniach, S. Chu, D. Herschlag, “Exploring the
folding landscape of a structured RNA,” Proc. Natl. Acad. Sci. USA 99, 155-160 (2002)
23. X. Zhuang, L. Bartley, H. Babcock, R. Russell, T. Ha, D. Herschlag, S. Chu, “A single-molecule
study of RNA catalysis and folding,” Science 288, 2048-2051 (2000)
24. X. Zhuang, T. Ha, H. Kim, T. Centner, S. Labeit, S. Chu, “Fluorescence quenching: a tool for singlemolecule
protein-folding study,” Proc. Natl. Acad. Sci. USA 97, 14241-14244 (2000)
25. T. Ha, X. Zhuang, H. Kim, J. Orr, J. Williamson, S. Chu, “Ligand-induced conformational changes
of single RNA molecules,” Proc. Natl. Acad. Sci. USA 96, 9077-9082 (1999)
26. S.-C. Hong, M. Oh-e, X. Zhuang, Y. R. Shen, J. J. Ge, F. W. Harris, S. Z. D. Cheng, “Orientation of
side chains and adsorbed liquid crystal molecules on a rubbed polyimide surface studied by optical
harmonic generation,” Phys. Rev. E 63, 0517061-7 (2001)
27. J. J. Ge, C. Y. Li, G.I. Xue, I. K. Mann, S. Z. D. Cheng, J. Z. Zhang, D. Zhang, S. Wang, F. W.
Harris, S.-C. Hong, X. Zhuang, Y. R. Shen, “Rubbing-induced molecular reorientation on an
alignment surface of an aromatic polyimide containing cyanobiphenyl side chains,” J. Am. Chem.
Soc. 123, 5768-5776 (2001)
28. X. Wei, X. Zhuang, D. Kim, S.-C. Hong, T. Goto, and Y. R. Shen, “Vibrational spectroscopy of
rubbed polymer surfaces,” Mole. Cryst. Liq. Cryst. 358, 103-108 (2001)
29. X. Wei, S. Hong, X. Zhuang, T. Goto, Y. R. Shen, “Nonlinear optical studies of liquid crystal
alignment on a rubber polyvinyl alcohol surface,” Phys. Rev. E 62, 5160-5172, (2000)
30. X. Wei, X. Zhuang, S. Hong, T. Goto, Y. R. Shen, “Sum-Frequency vibrational spectroscopic study
of a rubbed polymer surface,” Phys. Rev. Lett. 82, 4256-4259 (1999)
31. X. Zhuang, P. B. Miranda, D. Kim, Y. R. Shen, “Mapping molecular orientation and conformation at
interfaces by surface nonlinear optics,” Phys. Rev. B 59, 12632-12640 (1999)
32. T. Qian, X. Zhuang, Y. R. Shen, “Surface-monolayer-induced bulk alignment of liquid crystals:
from nematic to smectic-a phase,” Phys. Rev. E 59, 1873-1879 (1999)
33. X. Zhuang, R. Muenster, M. Jarasch, Y. R. Shen, “Dye-induced enhancement of optical nonlinearity
in liquid crystals and ordinary liquids,” Mole. Cryst. Liq. Cryst. 321, 165-172 (1998)
34. J. J. Ge, G.Xue, K. W. McCreight, S. Wang, F. W. Harris, S. Z. D. Cheng, X. Zhuang, S. Hong, Y.
R. Shen, “Surface studies of polyimide thin films via surface enhanced Raman scattering and second
harmonic generation,” Macromol. Rapid Comm. 19, 619-623 (1998)
35. R. Muenster, M. Jarasch, X. Zhuang, Y. R. Shen, “Enhanced optical Kerr effect of dye-doped
isotropic liquid,” Phys. Rev. Lett. 78, 42-45 (1997)
36. X. Zhuang and Y. R. Shen, “The application of nonlinear optics to the study of polymers at
interfaces,” Trends Polym. Sci. 4, 258-264 (1996)
37. A. Le Calvez, S. Montant, E. Freysz, A. Ducasse, X. Zhuang, Y. R. Shen, “Ultrafast orientation
dynamics of liquid crystals in smectic phase,” Chem. Phys. Lett. 258, 620-625 (1996)
38. X. Zhuang, H. S. Lackritz, and Y. R. Shen, “Photo-isomerization of polymer monolayers and multilayers
on water,” Chem. Phys. Lett. 246, 279-284 (1995)
39. X. Zhuang, D. Wilk, L. Marrucci, and Y. R. Shen, “Orientation of amphiphilic molecules on polar
substrates,” Phys. Rev. Lett. 75, 2144-2147 (1995)
40. X. Zhuang, L. Marrucci, D. Johannsmann, and Y. R. Shen, “Dependence of liquid crystal bulk
alignment on its surface monolayer,” Mole. Cryst. Liq. Cryst. 262, 35-43 (1995)
41. X. Zhuang, L. Marrucci, and Y. R. Shen, “Surface-monolayer-induced bulk alignment of liquid
crystals,” Phys. Rev. Lett. 73, 1513-1516 (1994)